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Product Info Summary
| SKU: | PA2217 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-AACT/SERPINA3 Antibody
View all Serpin A3/alpha 1-Antichymotrypsin Antibodies
SKU/Catalog Number
PA2217
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AACT/SERPINA3 Antibody catalog # PA2217. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AACT/SERPINA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2217)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human alpha 1 Antichymotrypsin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2217 is reactive to SERPINA3 in Human
Applications
PA2217 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
47.651kDa
Background of Serpin A3/alpha 1-Antichymotrypsin
Alpha 1-antichymotrypsin, also called serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 or GIG24 is an alpha globulin glycoprotein that is a member of the serpin superfamily. In humans, it is encoded by the SERPINA3 gene. This gene is mapped to 14q32.13. The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-AACT/SERPINA3 antibody, PA2217, Western blotting
WB: SMMC Cell Lysate
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Anti-AACT/SERPINA3 antibody, PA2217, IHC(P)
IHC(P): Human Liver Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For SERPINA3 (Source: Uniprot.org, NCBI)
Gene Name
SERPINA3
Full Name
Alpha-1-antichymotrypsin
Weight
47.651kDa
Superfamily
serpin family
Alternative Names
AACTMGC88254; ACTGIG25; alpha 1-Antichymotrypsin; alpha-1-antichymotrypsin; antitrypsin), member 3; Cell growth-inhibiting gene 24/25 protein; growth-inhibiting protein 24; growth-inhibiting protein 25; member 3; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 3; Serpin A3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) SERPINA3 AACT, ACT, GIG24, GIG25 serpin family A member 3 alpha-1-antichymotrypsin|cell growth-inhibiting gene 24/25 protein|growth-inhibiting protein 24|growth-inhibiting protein 25|serine (or cysteine) proteinase inhibitor, clade A, member 3|serpin A3|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINA3, check out the SERPINA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AACT/SERPINA3 Antibody (PA2217)
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No publications found for PA2217
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-AACT/SERPINA3 Antibody
Question
Here is the WB image, lot number and protocol we used for liver using anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
We are currently using anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-alpha 1 antichymotrypsin/SERPINA3 antibody (PA2217) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
I was wanting to use your anti-alpha 1 antichymotrypsin/SERPINA3 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
B. Roberts
Verified customer
Asked: 2018-11-20
Answer
You can see on the product datasheet, PA2217 anti-alpha 1 antichymotrypsin/SERPINA3 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-20
Question
I see that the anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-19
Question
My question regarding product PA2217, anti-alpha 1 antichymotrypsin/SERPINA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Johnson
Verified customer
Asked: 2018-01-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2217 anti-alpha 1 antichymotrypsin/SERPINA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-15
Question
I am looking for to test anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217 on human liver for research purposes, then I may be interested in using anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Huang
Verified customer
Asked: 2016-06-08
Answer
The products we sell, including anti-alpha 1 antichymotrypsin/SERPINA3 antibody PA2217, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-08
