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Product Info Summary
| SKU: | A02419-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Alpha 1 microglobulin/Ambp Antibody Picoband®
SKU/Catalog Number
A02419-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha 1 microglobulin/Ambp Antibody Picoband® catalog # A02419-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Alpha 1 microglobulin/Ambp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02419-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Alpha 1 microglobulin/Ambp recombinant protein (Position: A205-S349).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02419-4 is reactive to Ambp in Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.0 kDa
Background of Ambp
Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a fusion protein that undergoes proteolytic processing to generate two mature proteins: alpha-1-microglobulin (A1m) is a heme-binding plasma glycoprotein of the lipocalin superfamily of proteins that bind to hydrophobic molecules, whereas bikunin belongs to the superfamily of Kunitz-type protease inhibitors. The transgenic mice specifically lacking bikunin, but not A1m, exhibit female infertility and an increased sensitivity to lung metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02419-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat liver tissue, mouse kidney tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of Alpha 1 Microglobulin/Ambp using anti-Alpha 1 Microglobulin/Ambp antibody (A02419-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Microglobulin/Ambp antigen affinity purified polyclonal antibody (Catalog # A02419-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Microglobulin/Ambp at approximately 39 kDa. The expected band size for Alpha 1 Microglobulin/Ambp is at 39 kDa.
Specific Publications For Anti-Alpha 1 microglobulin/Ambp Antibody Picoband® (A02419-4)
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