Anti-Alpha 1 microglobulin/Ambp Antibody Picoband™

Product Name

Anti-Alpha 1 microglobulin/Ambp Antibody Picoband™

View all AMBP Antibodies

SKU/Catalog Number

A02419-4

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha 1 microglobulin/Ambp Antibody Picoband™ catalog # A02419-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Alpha 1 microglobulin/Ambp Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02419-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Alpha 1 microglobulin/Ambp recombinant protein (Position: A205-S349).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02419-4 is reactive to AMBP in Mouse, Rat

Applications

A02419-4 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.999kDa

Background of AMBP

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a fusion protein that undergoes proteolytic processing to generate two mature proteins: alpha-1-microglobulin (A1m) is a heme-binding plasma glycoprotein of the lipocalin superfamily of proteins that bind to hydrophobic molecules, whereas bikunin belongs to the superfamily of Kunitz-type protease inhibitors. The transgenic mice specifically lacking bikunin, but not A1m, exhibit female infertility and an increased sensitivity to lung metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Alpha 1 Microglobulin/Ambp using anti-Alpha 1 Microglobulin/Ambp antibody (A02419-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Microglobulin/Ambp antigen affinity purified polyclonal antibody (Catalog # A02419-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Microglobulin/Ambp at approximately 39 kDa. The expected band size for Alpha 1 Microglobulin/Ambp is at 39 kDa.

Gene/Protein Information For AMBP (Source: Uniprot.org, NCBI)

Alternative Names

A1M; alpha 1Microglobulin; alpha 1-Microglobulin; alpha-1-microglobulin/bikunin precursor; AMBP; bikunin; EDC1; HCP; HCPcomplex-forming glycoprotein heterogeneous in charge; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; ITILgrowth-inhibiting protein 19; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI AMBP A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI alpha-1-microglobulin/bikunin precursor protein AMBP|bikunin|complex-forming glycoprotein heterogeneous in charge|growth-inhibiting protein 19|inter-alpha-trypsin inhibitor light chain|protein HC|trypstatin|uristatin|uronic-acid-rich protein

For more info on AMBP, check out the AMBP Infographic

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In this infographic, you will see the following information for AMBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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