Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A04248-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband®
SKU/Catalog Number
A04248-2
Size
100 μg/vial
Form
Lyophilized
Description
SERPINF2 (alpha-2-antiplasmin) is a serpin that inactivates plasmin, positioning it in fibrinolysis/blood clot regulation; the page notes deficiency phenotypes associated with SERPINF2 mutations (hemorrhagic diathesis). Assay context: validated for ELISA, IHC, and WB in human samples, enabling coagulation-pathway profiling in plasma/tissues and orthogonal protein confirmation (research use). Often contrasted with extracellular-matrix proteolysis markers such as MMP9 because both influence tissue remodeling, but via distinct protease-control axes (fibrin vs ECM; interpretive, putative).
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04248-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Alpha 2 Antiplasmin/SERPINF2 recombinant protein (Position: K461-K491).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04248-2 is reactive to SERPINF2 in Human
Observed Molecular Weight
65 kDa
Calculated molecular weight
54.6 kDa
Background of SERPINF2
Alpha 2-antiplasmin (or α2-antiplasmin or plasmin inhibitor) is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene. This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04248-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human K562 whole cell, human HCCT tissue
IHC: human liver cancer tissue, human lymphoma tissue, human stomach cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HCCT tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 2 Antiplasmin/SERPINF2 antigen affinity purified polyclonal antibody (Catalog # A04248-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 2 Antiplasmin/SERPINF2 at approximately 65 kDa. The expected band size for Alpha 2 Antiplasmin/SERPINF2 is at 65 kDa.
Click image to see more details
IHC analysis of Alpha 2-Antiplasmin/SERPINF2 using anti-Alpha 2-Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2-Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2-Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® (A04248-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
