Anti-Alpha II-spectrin / Alpha-Fodrin / SPTAN1 Monoclonal Antibody

Product Name

Anti-Alpha II-spectrin / Alpha-Fodrin / SPTAN1 Monoclonal Antibody

View all Alpha Fodrin Antibodies

SKU/Catalog Number

M03831

Size

100ul

Description

Boster Bio Anti-Alpha II-spectrin / Alpha-Fodrin / SPTAN1 Monoclonal Antibody catalog # M03831. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha II-spectrin / Alpha-Fodrin / SPTAN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03831)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Immunogen

Recombinant construct containing the 19th and 20th spectrin repeats and C-terminal EF hand domains of human II spectrin (amino acids 2086-2445 from AAB41498.1) expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03831 is reactive to SPTAN1 in Human, Mouse, Rat

Applications

M03831 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

284.539kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:3,000. IF/ICC: 1:500.

Validation Images & Assay Conditions

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Western blot analysis of neural tissue and cell lysates using mouse mAb to II spectrin, M03831, in green. [1] protein standard, [2] rat whole brain, [3] rat spinal cord, [4] mouse whole brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, [10] C6 glioma cells. A prominent band at about 250-260 kDa represents the intact II spectrin heavy chain.

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Immunofluorescent image of rat cerebellum stained with M03831 antibody to II spectrin, dilution 1:2,000, in green and , chicken polyclonal antibody to GFAP, dilution 1:5,000, in red. The spectrin antibody stains the submembraneous cytoskeleton on neurons and strongly reveals the cell bodies and dendrites of Purkinje cells, while the GFAP antibody stains the processes of Bergmann glia and astrocytes.

Gene/Protein Information For SPTAN1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha-II spectrin; EIEE5; FLJ17738; FLJ44613; Fodrin alpha chain; NEAS; spectrin alpha chain, brain; spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spectrin, non-erythroid alpha chain; SPTA2 SPTAN1 DEE5, EIEE5, NEAS, SPTA2 spectrin alpha, non-erythrocytic 1 spectrin alpha chain, non-erythrocytic 1|alpha-II spectrin|alpha-fodrin|epididymis secretory sperm binding protein|fodrin alpha chain|spectrin, non-erythroid alpha chain|spectrin, non-erythroid alpha subunit

For more info on SPTAN1, check out the SPTAN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPTAN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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