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Product Info Summary
| SKU: | M02217-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody
View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies
SKU/Catalog Number
M02217-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody catalog # M02217-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02217-2)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
4A12
Isotype
IgG
Immunogen
Synthetic Peptide
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02217-2 is reactive to Amacr in Human, Mouse, Rat
Applications
M02217-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.704kDa
Background of alpha-Methylacyl-CoA Racemase/AMACR
LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000
IHC 1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of AMACR using Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2).
Immunohistochemical analysis of human-stomach-cancer tissue. Anti-AMACR at 1:200 (4°C
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Figure 3. Immunohistochemistry validation of AMACR using Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2).
Immunohistochemical analysis of human stomach tissue. Anti-AMACR at 1:200 (4°C
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Figure 4. Immunohistochemistry validation of AMACR using Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2).
Immunohistochemical analysis of mouse kidney tissue. Anti-AMACR at 1:200 (4°C
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Figure 5. Immunohistochemistry validation of AMACR using Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2).
Immunohistochemical analysis of rat kidney tissue. Anti-AMACR at 1:200 (4°C
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Figure 6. Immunohistochemistry validation of AMACR using Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2).
Immunohistochemistry (IHC) staining of Mouse prostate adenocarcinoma tissue
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Figure 1. Western blotting validation for Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody M02217-2
Western blot (WB) analysis of AMACR monoclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Amacr (Source: Uniprot.org, NCBI)
Gene Name
Amacr
Full Name
Alpha-methylacyl-CoA racemase
Weight
41.704kDa
Superfamily
CoA-transferase III family
Alternative Names
2-methylacyl-CoA racemase; alpha-Methylacyl-CoA Racemase; alphaMethylacylCoARacemase; AMACR; AMACRD; CBAS4; EC 5.1.99.4; RACE; RM Amacr|Mac, Macr1|alpha-methylacyl-CoA racemase|alpha-methylacyl-CoA racemase|2-arylpropionyl-CoA epimerase|2-methylacyl-CoA racemase|alpha-methylacyl-Coenzyme A racemase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Amacr, check out the Amacr Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Amacr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-methylacyl-CoA racemase AMACR Monoclonal Antibody (M02217-2)
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