Anti-alpha-Tubulin Tuba1b Antibody

Product Name

Anti-alpha-Tubulin Tuba1b Antibody

View all TUBA1B Antibodies

SKU/Catalog Number

A08382

Size

200ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-alpha-Tubulin Tuba1b Antibody (Catalog # A08382). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store Anti-Tubulin Loading Control Antibody at -20°C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-alpha-Tubulin Tuba1b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08382)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08382 is reactive to TUBA1B in Human, Mouse, Rat

Calculated molecular weight

50.2 kDa

Background of TUBA1B

Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08382 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:90,000
IHC: 1:500 - 1:2,000
IF Microscopy: 1:500 - 1:2,000
WB: 1:1,000 - 1:5,000
Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Validation Images & Assay Conditions

See full target information TUBA1B

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