Anti-Alpha-tubulin TUBA3C Antibody

Product Name

Anti-Alpha-tubulin TUBA3C Antibody

View all TUBA3C Antibodies

SKU/Catalog Number

A17842-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Alpha-tubulin TUBA3C Antibody (Catalog # A17842-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Alpha-tubulin TUBA3C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17842-1)

Host

Rabbit

Contents

Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin.

Cross-reactivity

Alpha-tubulin antibody is human, mouse and rat reactive.

Reactive Species

A17842-1 is reactive to TUBA3C in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Background of TUBA3C

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A17842-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TUBA3C

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis