Anti-ALPP/ALPPL2 Antibody

Boster Bio Anti-ALPP/ALPPL2 Antibody catalog # A01718-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A01718-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-ALPP/ALPPL2 Antibody

See all Alkaline Phosphatase/ALPP products

SKU/Catalog Number

A01718-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ALPP/ALPPL2 Antibody catalog # A01718-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALPP/ALPPL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01718-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01718-1 is reactive to ALPP in Human, Mouse

Applications

A01718-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ALPP (Source: Uniprot.Org, NCBI)

Uniprot ID

P05187

Gene ID

250

Gene Name

ALPP

Full Name

Alkaline phosphatase, placental type

Weight

57.954kDa

Superfamily

alkaline phosphatase family

Alternative Names

Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP ALPP ALP, ALPI, IAP, PALP, PLAP, PLAP-1 alkaline phosphatase, placental alkaline phosphatase, placental type|Intestinal alkaline phosphatase|Intestinal-type alkaline phosphatase|alkaline phosphatase Regan isozyme|alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ALPP, check out the ALPP Infographic

ALPP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ALPP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-ALPP/ALPPL2 Antibody

Question

I am looking for to test anti-ALPP/ALPPL2 antibody A01718-1 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-ALPP/ALPPL2 antibody A01718-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

The products we sell, including anti-ALPP/ALPPL2 antibody A01718-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-02

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-ALPP/ALPPL2 antibody A01718-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-08

Question

I see that the anti-ALPP/ALPPL2 antibody A01718-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-13

Question

Would anti-ALPP/ALPPL2 antibody A01718-1 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

According to the expression profile of cervix carcinoma, ALPP is highly expressed in cervix carcinoma. So, it is likely that anti-ALPP/ALPPL2 antibody A01718-1 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-10-31

Question

Can you help my question with product A01718-1, anti-ALPP/ALPPL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01718-1 anti-ALPP/ALPPL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-12

Question

I was wanting to use your anti-ALPP/ALPPL2 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

You can see on the product datasheet, A01718-1 anti-ALPP/ALPPL2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-30

Question

Would anti-ALPP/ALPPL2 antibody A01718-1 work on bovine WB with cervix placenta?

Z. Li

Verified customer

Asked: 2018-11-08

Answer

Our lab technicians have not validated anti-ALPP/ALPPL2 antibody A01718-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ALPP/ALPPL2 antibody A01718-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-08

Question

Is this A01718-1 anti-ALPP/ALPPL2 antibody reactive to the isotypes of ALPP?

Verified Customer

Verified customer

Asked: 2018-05-15

Answer

The immunogen of A01718-1 anti-ALPP/ALPPL2 antibody is Synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-15

Question

Does A01718-1 anti-ALPP/ALPPL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

V. Edwards

Verified customer

Asked: 2018-04-23

Answer

It shows on the product datasheet, A01718-1 anti-ALPP/ALPPL2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-23

Question

Our lab were well pleased with the WB result of your anti-ALPP/ALPPL2 antibody. However we have seen positive staining in cervix placenta cell membrane using this antibody. Is that expected? Could you tell me where is ALPP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

From what I have seen in literature, cervix placenta does express ALPP. Generally ALPP expresses in cell membrane. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-04-20

Question

Is a blocking peptide available for product anti-ALPP/ALPPL2 antibody (A01718-1)?

D. Carter

Verified customer

Asked: 2017-12-13

Answer

We do provide the blocking peptide for product anti-ALPP/ALPPL2 antibody (A01718-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-13

Question

We are currently using anti-ALPP/ALPPL2 antibody A01718-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?

D. Jha

Verified customer

Asked: 2016-01-07

Answer

The anti-ALPP/ALPPL2 antibody (A01718-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ALPP/ALPPL2 antibody A01718-1. Let me know if you need anything else.

G. Mangal

Verified customer

Asked: 2014-03-31

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-31

Question

Do you have a BSA free version of anti-ALPP/ALPPL2 antibody A01718-1 available?

K. Singh

Verified customer

Asked: 2014-03-26

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ALPP/ALPPL2 antibody A01718-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-03-26

Question

Our lab want to know about using your anti-ALPP/ALPPL2 antibody for intra-golgi traffic studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.

F. Rodriguez

Verified customer

Asked: 2013-10-22

Answer

Thank you for your inquiry. This A01718-1 anti-ALPP/ALPPL2 antibody is validated on 293 cells. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-10-22

Question

We have observed staining in human cervix carcinoma. What should we do? Is anti-ALPP/ALPPL2 antibody supposed to stain cervix carcinoma positively?

J. Lewis

Verified customer

Asked: 2013-08-21

Answer

From what I have seen in literature cervix carcinoma does express ALPP. From what I have seen in Uniprot.org, ALPP is expressed in placenta, cervix placenta, cervix carcinoma, among other tissues. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-08-21

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A01718-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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