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Product Info Summary
| SKU: | A00437-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-AMCase/CHIA Antibody Picoband®
SKU/Catalog Number
A00437-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMCase/CHIA Antibody Picoband® catalog # A00437-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. CHIA (acidic mammalian chitinase; AMCase) is described as a chitin-degrading enzyme (relevant to fungi/arthropod/nematode chitin) and is also described to stimulate IL-13 expression, with gene variation linked to asthma susceptibility. Assay context: validated for ELISA, IHC, and Western blot in Human/Mouse (observed ~52 kDa; on-page positive controls include mouse stomach lysate and human stomach cancer tissue); airway inflammation interpretations are often contrasted with pro-inflammatory IL-1 axis markers such as IL1B and counter-regulatory IL-1 family cytokines such as IL-37 (IL1F7) (panel context; putative).
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AMCase/CHIA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00437-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AMCase/CHIA recombinant protein (Position: L228-A476).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00437-1 is reactive to CHIA in Human, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.3 kDa
Background of CHIA
The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00437-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
"Western blot, 0.5-1 μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human
ELISA, 0.1-0.5 μg/ml, -
"
Positive Control
WB: mouse stomach tissue
IHC: human stomach cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMCase/CHIA antigen affinity purified polyclonal antibody (A00437-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AMCase/CHIA at approximately 52 kDa. The expected band size for AMCase/CHIA is at 52 kDa.
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IHC analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1).
AMCase/CHIA was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AMCase/CHIA Antibody (A00437-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-AMCase/CHIA Antibody Picoband® (A00437-1)
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