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Product Info Summary
| SKU: | A05093 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Ameloblastin AMBN Antibody
SKU/Catalog Number
A05093
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ameloblastin AMBN Antibody (Catalog# A05093). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ameloblastin AMBN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05093)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Ameloblastin. Immunogen sequence location: 275 - 324
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05093 is reactive to AMBN in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05093 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells, using Ameloblastin antibody.
Specific Publications For Anti-Ameloblastin AMBN Antibody (A05093)
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4 Customer Q&As for Anti-Ameloblastin AMBN Antibody
Question
We are currently using anti-Ameloblastin antibody A05093 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-Ameloblastin antibody (A05093) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
I was wanting to use your anti-Ameloblastin antibody for WB for human molar on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human molar identification?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can see on the product datasheet, A05093 anti-Ameloblastin antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human molar in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-24
Question
Is this A05093 anti-Ameloblastin antibody reactive to the isotypes of AMBN?
K. Walker
Verified customer
Asked: 2019-04-24
Answer
The immunogen of A05093 anti-Ameloblastin antibody is A peptide derived from human Ameloblastin. Immunogen sequence location: 275 - 324. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-24
Question
Would A05093 anti-Ameloblastin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
It shows on the product datasheet, A05093 anti-Ameloblastin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-16
