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Product Info Summary
| SKU: | PB9738 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-AMPK beta 1/PRKAB1 Antibody Picoband®
SKU/Catalog Number
PB9738
PB0722 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
PRKAB1 encodes the AMPK β1 regulatory subunit within the heterotrimeric AMP-activated protein kinase complex, a core cellular energy sensor coordinating catabolic vs anabolic balance under energy stress. Assay context: human-reactive antibody tested for IF, ICC, and WB. Often interpreted with metabolic-state enzymes such as ABHD5 (lipid mobilization) or mitochondrial metabolism markers like ALDH6A1 when linking signaling state to substrate flux (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK beta 1/PRKAB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9738)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 1, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9738 is reactive to PRKAB1 in Human
Observed Molecular Weight
38 kDa
Calculated molecular weight
30.4 kDa
Background of PRKAB1
5'-AMP-activated protein kinase subunit beta-1 is an enzyme that in humans is encoded by the PRKAB1 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9738 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, PANC Whole Cell, K562 Whole Cell
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: JURKAT Whole Cell Lysate,
Lane 2: PANC Whole Cell Lysate,
Lane 3: K562 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 1 at approximately 38 kDa. The expected band size for AMPK beta 1 is at 30 kDa.
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IF analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738).
AMPK beta 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AMPK beta 1 Antibody (PB9738) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-AMPK beta 1/PRKAB1 Antibody Picoband® (PB9738)
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3 Customer Q&As for Anti-AMPK beta 1/PRKAB1 Antibody Picoband®
Question
We are currently using anti-AMPK beta 1/PRKAB1 antibody PB9738 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-AMPK beta 1/PRKAB1 antibody (PB9738) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
I was wanting to use your anti-AMPK beta 1/PRKAB1 antibody for WB for human lung muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung muscle identification?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, PB9738 anti-AMPK beta 1/PRKAB1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-AMPK beta 1/PRKAB1 antibody PB9738 work for WB with lung muscle?
C. Carter
Verified customer
Asked: 2015-12-03
Answer
According to the expression profile of lung muscle, PRKAB1 is highly expressed in lung muscle. So, it is likely that anti-AMPK beta 1/PRKAB1 antibody PB9738 will work for WB with lung muscle.
Boster Scientific Support
Answered: 2015-12-03
