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Product Info Summary
| SKU: | A30557 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AN30A Antibody
SKU/Catalog Number
A30557
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AN30A Antibody (Catalog# A30557). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AN30A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30557)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AN30A. Immunogen sequence location: 1041-1090
Reactive Species
A30557 is reactive to ANKRD30A in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
158.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30557 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using ANKRD30A Polyclonal Antibody diluted at 1:2000.
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Western blot analysis of lysates from 293 and COLO cells, using AN30A Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-AN30A Antibody (A30557)
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1 Customer Q&As for Anti-AN30A Antibody
Question
We are currently using anti-AN30A antibody A30557 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
L. Baker
Verified customer
Asked: 2019-01-07
Answer
The anti-AN30A antibody (A30557) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-07
