Anti-ANAPC5/Apc5 Antibody

Product Name

Anti-ANAPC5/Apc5 Antibody

View all Apc5 Antibodies

SKU/Catalog Number

A08280

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ANAPC5/Apc5 Antibody (Catalog # A08280). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ANAPC5/Apc5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08280)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ANAPC5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08280 is reactive to ANAPC5 in Human

Applications

A08280 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

85.077kDa

Background of Apc5

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Yu H., Science 279:1219-1222(1998).
Bechtel S., BMC Genomics 8:399-399(2007).
Kraft C., EMBO J. 22:6598-6609(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody A08280.

Gene/Protein Information For ANAPC5 (Source: Uniprot.org, NCBI)

Alternative Names

anaphase promoting complex subunit 5; anaphase-promoting complex subunit 5; APC5Cyclosome subunit 5 ANAPC5 APC5 anaphase promoting complex subunit 5 anaphase-promoting complex subunit 5|cyclosome subunit 5

For more info on ANAPC5, check out the ANAPC5 Infographic

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In this infographic, you will see the following information for ANAPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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