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- Table of Contents
ANGPT1 antibody (A00853) performs well in ELISA, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody (A00853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human ANGPT1 protein 1ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody (Catalog # A00853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human ANGPT1 protein 1ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody (Catalog # A00853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.
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Anti-Angiopoietin-1/ANGPT1 Antibody Picoband®
ANGPT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, WB |
Tested Applications
ANGPT1 antibody was positively detected by WB in:
recombinant human ANGPT1 protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
ELISA
0.1-0.5μg/ml
-
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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ELISA
Human
Recommend
Bovine
Predicted
Monkey
Predicted
Rat
Predicted
Rabbit
Predicted
Dog
Predicted
Mouse
Predicted
Sheep
Predicted
Product Information
Product Name
Anti-Angiopoietin-1/ANGPT1 Antibody Picoband®
View all ANGPT1 Antibodies
View all ANGPT1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
57.5 kDa
Background of ANGPT1
Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietin 1 is mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Cite This Product
Anti-Angiopoietin-1/ANGPT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00853)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Monkey
100%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Dog
95%
High Homology
Mouse
92%
High Homology
Sheep
92%
High Homology
Zebrafish
55%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANGPT1 antibody
Publications
Application:
Species:
Expressions of Angiopoietin-1, Angiopoietin-2, and Tie2 and Their Roles in Rat Renal Allografts With Chronic Allograft Nephropathy
Published: 2008-10-22
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 1.03
Species: Rat
PMID: 18929864
Rapamycin and Cyclosporine Have Different Effects on Expression of ang-1 and ang-2 and Tie2 in Rat Renal Allograft With Chronic Allograft Nephropathy
Published: 2008-10-22
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 1.03
Species: Rat
PMID: 18929866
Immunohistochemical and in-situ hybridization study of hypoxia inducible factor-1α and angiopoietin-1 in a rabbit model of mandibular distraction osteogenesis
Published: 2008-03-12
Journal: INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
Application: IHC
Impact Factor: 1.23
Species: Rabbit
PMID: 18339517
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Product Promise
We promise Anti-Angiopoietin-1/ANGPT1 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00853
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026