Anti-Angiopoietin 1/ANGPT1 Antibody Picoband®

Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human Angiopoietin1 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 39 kDa. The expected band size for Angiopoietin 1 is at 39 kDa.

Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 65 kDa. The expected band size for Angiopoietin 1 is at 57 kDa.

(A) In the schematic brain diagram, the orange area represents the infarct area. Red boxes indicate the regions where immunofluorescence images were taken. The left side shows the ingrowth of lymphatic vessels on day 28, past stroke. Scale bar: 50 μm. (B) The distribution of meningeal lymphatic vessels on day 3 post-stroke in young FTG mice. The white arrow indicates the growth of lymphatic vessels. R: right side, L: left side. Scale bar: 1,000 μm. (C) Representative Western blot images of VEGF/GAPDH and Ang1/GAPDH in the cortex on day 28 post-stroke. (D) Quantification of VEGF/GAPDH and Ang1/GAPDH relative expression on day 28 post-stroke ( n = 5 per group). (E) Representative Western blot images of PROX1/GAPDH, Lyve-1/GAPDH, and VEGFC/β-actin in the cortex on day 28 post-stroke ( n = 5 per group). (F) Quantification of PROX1/GAPDH, Lyve-1/GAPDH, and VEGFC/β-actin relative expression on day 28 post-stroke.* P < 0.05, ** indicates a p- value of < 0.01 in the aged-FTG vs. young-FTG group.
Index in PubMed under a CC BY license. PMID: 39371609

Hyperoxia down-regulated pulmonary Twist1, Tie1, Tie2, and Ang1 mRNAs, while up-regulated Ang2 mRNA. Lungs from all different groups were excised, total RNA was isolated, and Twist1 (A) , Tie1 (B) , Tie2 (C) , Ang1 (D) , and Ang2 (E) mRNA levels were determined by real time-PCR following cDNA synthesis, as described in the Materials and Methods section ( n = 6/group). * P < 0.05, ** P < 0.01.
Index in PubMed under a CC BY license. PMID: 32391293

Hyperoxia reduced pulmonary Twist1, Tie1, Tie2, and Ang1 protein levels, while up-regulated Ang2 protein level. The lung homogenates prepared from different groups of newborn rats were subjected to western blots analysis. Western blots showed the expression of Twist1 (A,D) , Ang1/2 (A,C) , Tie1 (B,F) , pTie2/Tie2 (B,E) in the lungs. The densitometric intensities of these proteins normalized to Gapdh were quantified and shown separately ( n = 8–14/group). * P < 0.05, ** P < 0.01.
Index in PubMed under a CC BY license. PMID: 32391293