Click image to see more details
Product Info Summary
| SKU: | M00853 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00853
BM4539 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody catalog # M00853. Tested in WB application. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00853)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FGC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Angiopoietin 1
Reactive Species
M00853 is reactive to ANGPT1 in Human, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
57.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00853 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human Hela whole cell, human HEL whole cell, human K562 whole cell, human Caco-2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Angiopoietin-1/ANGPT1 using anti-Angiopoietin-1/ANGPT1 antibody (M00853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1/ANGPT1 antigen affinity purified monoclonal antibody (M00853) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1/ANGPT1 at approximately 70 kDa. The expected band size for Angiopoietin-1/ANGPT1 is at 58 kDa.
Specific Publications For Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody (M00853)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody
Question
Is this M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody reactive to the isotypes of ANGPT1?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The immunogen of M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Angiopoietin 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-01
Question
Does M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
You can see on the product datasheet, M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-09
Question
Does anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 work on monkey WB with cauda epididymis?
L. Moore
Verified customer
Asked: 2013-12-23
Answer
Our lab technicians have not validated anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cauda epididymis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-23
