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Product Info Summary
| SKU: | M02103 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody
SKU/Catalog Number
M02103
BM4639 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody catalog # M02103. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02103)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GGO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Angiotensinogen
Reactive Species
M02103 is reactive to AGT in Human
Observed Molecular Weight
53 kDa
Calculated molecular weight
53.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02103 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of Angiotensinogen expression in 293T cell lysate.
Specific Publications For Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody (M02103)
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3 Customer Q&As for Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody
Question
Will M02103 anti-Angiotensinogen Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
As indicated on the product datasheet, M02103 anti-Angiotensinogen Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
Is there a BSA free version of anti-Angiotensinogen Rabbit Monoclonal antibody M02103 available?
S. Zhao
Verified customer
Asked: 2019-11-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Angiotensinogen Rabbit Monoclonal antibody M02103 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-26
Question
Would anti-Angiotensinogen Rabbit Monoclonal antibody M02103 work for IP with serum?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to the expression profile of serum, AGT is highly expressed in serum. So, it is likely that anti-Angiotensinogen Rabbit Monoclonal antibody M02103 will work for IP with serum.
Boster Scientific Support
Answered: 2019-10-04
