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- Table of Contents
ANLN antibody (A03997-1) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of U251 cells using anti-Anillin/ANLN antibody (A03997-1).
Overlay histogram showing U251 cells stained with A03997-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Anillin/ANLN Antibody (A03997-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with A03997-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Anillin/ANLN Antibody (A03997-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Anillin/ANLN Antibody Picoband®
ANLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, ICC, WB |
Tested Applications
ANLN antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANLN antibody was positively detected by ICC/IF in:
U2OS cell
ANLN antibody was positively detected by IP in:
U251 cell
ANLN antibody was positively detected by FC in:
U251 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence
5 μg/ml
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Monkey
Predicted
Dog
Predicted
Product Information
Product Name
Anti-Anillin/ANLN Antibody Picoband®
View all ANLN Antibodies
View all ANLN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Anillin/ANLN, which shares 96.4% amino acid (aa) sequence identity with both mouse and rat Anillin/ANLN.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
124.2 kDa
Background of ANLN
Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and cytokinesis. This gene is mapped to 7p14.2. The ANLN gene in humans and the scraps gene in Drosophila encode Anillin. The human anillin cDNA, located on Chr7, encodes a 1,125–amino acid protein with a predicted molecular mass of 124 kD and a pI of 8.1. The mouse anillin gene is located on Chr9. This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-Anillin/ANLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03997-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Dog
96%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANLN antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ANLN antibody
Immunoprecipitation (IP) protocol for ANLN antibody
Flow Cytometry (FC) protocol for ANLN antibody
Publications
Application:
Species:
ANLN: A New Hub in Glutamine Metabolism of Lung Adenocarcinoma by scRNA-Seq and Machine Learning
Published: 2026-07-16
Journal: ONCOLOGY RESEARCH
Application: IHC
Impact Factor: 4.60
Species: Human
PMID: 42500572
A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer
Published: 2025-02-19
Journal: Cell Death & Disease
Application: WB
Impact Factor: 9.60
Species: Human
PMID: 39971925
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Product Promise
We promise Anti-Anillin/ANLN Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A03997-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026