Anti-ANKRD30A/Ny Br 1 Antibody

ANKRD30A antibody

Boster Bio Anti-ANKRD30A/Ny Br 1 Antibody catalog # A12867. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12867
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-ANKRD30A/Ny Br 1 Antibody

View all ANKRD30A Antibodies

SKU/Catalog Number

A12867

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ANKRD30A/Ny Br 1 Antibody catalog # A12867. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ANKRD30A/Ny Br 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12867)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2H4

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human ANKRD30A. at AA range: 1010-1090

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12867 is reactive to ANKRD30A in Human

Applications

A12867 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

158.835kDa

Background of ANKRD30A

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

Gene/Protein Information For ANKRD30A (Source: Uniprot.org, NCBI)

Gene Name

ANKRD30A

Full Name

Ankyrin repeat domain-containing protein 30A

Weight

158.835kDa

Alternative Names

Ankyrin repeat domain-containing protein 30A ANKRD30A NY-BR-1 ankyrin repeat domain 30A ankyrin repeat domain-containing protein 30A|serologically defined breast cancer antigen NY-BR-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ANKRD30A, check out the ANKRD30A Infographic

ANKRD30A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANKRD30A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ANKRD30A/Ny Br 1 Antibody

Question

Would anti-ANKRD30A/Ny Br 1 antibody A12867 work on pig WB with epithelium of mammary gland?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

Our lab technicians have not tested anti-ANKRD30A/Ny Br 1 antibody A12867 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ANKRD30A/Ny Br 1 antibody A12867 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig epithelium of mammary gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of mammary gland using anti-ANKRD30A/Ny Br 1 antibody A12867. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-11

Question

I have a question about product A12867, anti-ANKRD30A/Ny Br 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Moore

Verified customer

Asked: 2017-07-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12867 anti-ANKRD30A/Ny Br 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-17

Question

Is this A12867 anti-ANKRD30A/Ny Br 1 antibody reactive to the isotypes of ANKRD30A?

T. Kulkarni

Verified customer

Asked: 2013-10-25

Answer

The immunogen of A12867 anti-ANKRD30A/Ny Br 1 antibody is Synthesized peptide derived from the C-terminal region of human ANKRD30A. at AA rangle: 1010-1090. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-10-25

Order DetailsPrice
A12867

100uL

$399

Free Secondary Antibody

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A12867
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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