Anti-ANKRD30A/Ny Br 1 Antibody

Product Name

Anti-ANKRD30A/Ny Br 1 Antibody

View all ANKRD30A Antibodies

SKU/Catalog Number

A12867

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ANKRD30A/Ny Br 1 Antibody catalog # A12867. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ANKRD30A/Ny Br 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12867)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2H4

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human ANKRD30A. at AA range: 1010-1090

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12867 is reactive to ANKRD30A in Human

Applications

A12867 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

158.835kDa

Background of ANKRD30A

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of specific cells using ANKRD30A Polyclonal antibody.

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Western blot analysis of lysates from 293 and COLO cells, using AN30A Antibody. The lane on the right is blocked with the synthesized peptide.

Gene/Protein Information For ANKRD30A (Source: Uniprot.org, NCBI)

Alternative Names

Ankyrin repeat domain-containing protein 30A ANKRD30A NY-BR-1 ankyrin repeat domain 30A ankyrin repeat domain-containing protein 30A|serologically defined breast cancer antigen NY-BR-1

For more info on ANKRD30A, check out the ANKRD30A Infographic

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In this infographic, you will see the following information for ANKRD30A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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