Click image to see more details
Product Info Summary
| SKU: | DZ41074 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | African malaria mosquito |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-African malaria mosquito CTL4 Antibody
SKU/Catalog Number
DZ41074
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-CTL4 Antibody catalog # DZ41074. This antibody reacts with African malaria mosquito.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-African malaria mosquito CTL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41074)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41074 is reactive to CTL4 in African malaria mosquito
Observed Molecular Weight
20 kDa
Application & Images
Applications
DZ41074 is guaranteed for WB Boster Guarantee
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CTL4 using anti-CTL4 antibody (DZ41074).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mosquito hemolymph β-galactosidase KD lysates,
Lane 2: mosquito hemolymph CTL4 KD lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-CTL4 antigen affinity purified monoclonal antibody (DZ41074) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody at a dilution of 1:15000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for CTL4 at approximately 15 kDa. The expected band size for CTL4 is at 20 kDa.
Specific Publications For Anti-African malaria mosquito CTL4 Antibody (DZ41074)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-African malaria mosquito CTL4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
1 Reviews For Anti-African malaria mosquito CTL4 Antibody
The CTL4 antibody was efficiently used to detect CTL4 protein secreted in the mosquito hemolymph. The protein migrates at ~15 kDa in SDS-PAGE.
Excellent
| SKU | DZ41074 |
|---|---|
| Application | Western Blot |
| Sample | β-galactosidase gene knockdown mosquito hemolymph, CTL4 knockdown mosquito hemolymph |
| Primary Incubation | +4°C overnight |
| Secondary Incubation | For 1 hour at room temperature |
| Tertiary Incubation | 1:1000 |
| Detection | Clarity Max western ECL substrate |
| Results Summary | Image showing the specificity of CTL4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CTL4 gene. |
Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut
Verified customer
Submitted 2025-04-08
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
