Anti-ANP/NPPA Antibody Picoband™

Atrial Natriuretic Peptide/ANP antibody

Boster Bio Anti-ANP/NPPA Antibody Picoband™ catalog # PB9295. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9295
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-ANP/NPPA Antibody Picoband™

View all Atrial Natriuretic Peptide/ANP Antibodies

SKU/Catalog Number

PB9295

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ANP/NPPA Antibody Picoband™ catalog # PB9295. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ANP/NPPA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9295)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ANP, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9295 is reactive to NPPA in Human, Mouse, Rat

Applications

PB9295 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

16.396kDa

Background of Atrial Natriuretic Peptide/ANP

Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For NPPA (Source: Uniprot.org, NCBI)

Gene Name

NPPA

Full Name

Natriuretic peptides A

Weight

16.396kDa

Superfamily

natriuretic peptide family

Alternative Names

ANF; ANP; ANPatriopeptin; ATFB6; atrial natriuretic factor; Atrial Natriuretic Peptide; Cardionatrin; CDD-ANF; natriuretic peptide A; natriuretic peptide precursor A; NPPA; PND; PNDcardionatrin; prepronatriodilatin NPPA ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND natriuretic peptide A natriuretic peptides A|atrial natriuretic factor prohormone|atrial natriuretic peptide prohormone|atriopeptigen|atriopeptin|cardiodilatin-related peptide|cardionatrin|natriuretic peptide precursor A variant 1|preproANP|preproCDD-ANF|prepronatriodilatin|proANF|proANP

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NPPA, check out the NPPA Infographic

NPPA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NPPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9295 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Localization of corin and atrial natriuretic peptide expression in human renal segments

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6 Customer Q&As for Anti-ANP/NPPA Antibody Picoband™

Question

My question regarding product PB9295, anti-ANP/NPPA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9295 anti-ANP/NPPA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-01

Question

We are currently using anti-ANP/NPPA antibody PB9295 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-ANP/NPPA antibody (PB9295) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

Is this PB9295 anti-ANP/NPPA antibody reactive to the isotypes of NPPA?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

The immunogen of PB9295 anti-ANP/NPPA antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ANP(124-151aa SLRRSSCFGGRMDRIGAQSGLGCNSFRY), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-22

Question

I see that the anti-ANP/NPPA antibody PB9295 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-16

Question

Is PB9295 conjugated or not?

Verified customer

Asked: 2019-06-26

Answer

The Anti-ANP/NPPA Antibody Picoband™ (PB9295) is not conjugated.

Boster Scientific Support

Answered: 2019-06-26

Question

Does anti-ANP/NPPA antibody PB9295 work on dog WB with prostate?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

Our lab technicians have not validated anti-ANP/NPPA antibody PB9295 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ANP/NPPA antibody PB9295 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog prostate in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-15

Order DetailsPrice
PB9295

100μg

$370
PB9295-10ug

10μg sample (liquid)

$99
PB9295-Biotin

100 μg Biotin conjugated

$570
PB9295-Cy3

100 μg Cy3 conjugated

$570
PB9295-Dylight488

100 μg Dylight488 conjugated

$570
PB9295-Dylight550

100 μg Dylight550 conjugated

$570
PB9295-Dylight594

100 μg Dylight594 conjugated

$570
PB9295-FITC

100 μg FITC conjugated

$570
PB9295-HRP

100 μg HRP conjugated

$570
PB9295-APC

100 μg APC conjugated

$670
PB9295-PE

100 μg PE conjugated

$670
PB9295-carrier-free

Carrier Free

$370

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PB9295
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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