Anti-ANP/NPPA Antibody Picoband®

Product Name

Anti-ANP/NPPA Antibody Picoband®

View all NPPA Antibodies

SKU/Catalog Number

PB9295
PB0275 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ANP/NPPA Antibody Picoband® catalog # PB9295. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ANP/NPPA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9295)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ANP, different from the related mouse and rat sequences by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9295 is reactive to NPPA in Human, Mouse, Rat

Observed Molecular Weight

17 kDa

Calculated molecular weight

16.4 kDa

Background of NPPA

Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9295 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Positive Control

WB: Rat Cardiac Muscle Tissue, Mouse Cardiac Muscle Tissue

Validation Images & Assay Conditions

See full target information NPPA

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