Anti-APC4 ANAPC4 Antibody

Product Name

Anti-APC4 ANAPC4 Antibody

View all ANAPC4 Antibodies

SKU/Catalog Number

A06703

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-APC4 ANAPC4 Antibody (Catalog # A06703). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

APC4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-APC4 ANAPC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06703)

Host

Rabbit

Contents

APC4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. The immunogen is located within amino acids 40 - 90 of APC4.

Reactive Species

A06703 is reactive to ANAPC4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

92.1 kDa

Background of ANAPC4

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06703 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APC4 antibody can be used for detection of APC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ANAPC4

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