Product Info Summary
SKU: | A06703 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-APC4 ANAPC4 Antibody
SKU/Catalog Number
A06703
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC4 ANAPC4 Antibody (Catalog # A06703). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APC4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC4 ANAPC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06703)
Host
Rabbit
Contents
APC4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
SK7
Isotype
IgG
Immunogen
APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. The immunogen is located within amino acids 40 - 90 of APC4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.
Reactive Species
A06703 is reactive to ANAPC4 in Human, Mouse, Rat
Applications
A06703 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
92.116kDa
Background of Apc4
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC4 antibody can be used for detection of APC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of APC4 in mouse liver tissue lysate with APC4 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunohistochemistry of APC4 in rat liver tissue with APC4 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ANAPC4 (Source: Uniprot.org, NCBI)
Gene Name
ANAPC4
Full Name
Anaphase-promoting complex subunit 4
Weight
92.116kDa
Superfamily
APC4 family
Alternative Names
anaphase promoting complex subunit 4; anaphase-promoting complex subunit 4; APC4Cyclosome subunit 4 ANAPC4 APC4 anaphase promoting complex subunit 4 anaphase-promoting complex subunit 4|cyclosome subunit 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANAPC4, check out the ANAPC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANAPC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC4 ANAPC4 Antibody (A06703)
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