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Product Info Summary
| SKU: | A08394 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-APEH Antibody
SKU/Catalog Number
A08394
Size
100ul
Form
Liquid
Description
Boster Bio Anti-APEH Antibody catalog # A08394. Tested in WB,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APEH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08394)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human APEH(NP_001631.3).
Reactive Species
A08394 is reactive to APEH in Human, Mouse, Rat
Calculated molecular weight
81.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08394 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IP: 1:20-1:50
Validation Images & Assay Conditions
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Western blot analysis of various lysates, using APEH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug APEH antibody . Western blot was performed from the immunoprecipitate using APEH antibody at a dilution of 1:1000.
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Western blot analysis of extracts from wild type and APEH knockout 293T cells, using APEH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Specific Publications For Anti-APEH Antibody (A08394)
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