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Product Info Summary
| SKU: | M07803 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody
SKU/Catalog Number
M07803
BM5104 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody catalog # M07803. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07803)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABBD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Apg10 (Atg10)
Reactive Species
M07803 is reactive to ATG10 in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
25.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07803 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Apg10 using anti-Apg10 antibody (M07803).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg10 antigen affinity purified monoclonal antibody (Catalog # M07803) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg10 at approximately 28 kDa. The expected band size for Apg10 is at 25 kDa.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
Specific Publications For Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody (M07803)
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Customer Q&As
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7 Customer Q&As for Anti-Apg10 (Atg10) Rabbit Monoclonal Antibody
Question
We want to test anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 on human testis for research purposes, then I may be interested in using anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The products we sell, including anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-25
Question
Is a blocking peptide available for product anti-Apg10 (Atg10) Rabbit Monoclonal antibody (M07803)?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We do provide the blocking peptide for product anti-Apg10 (Atg10) Rabbit Monoclonal antibody (M07803). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-30
Question
I was wanting to use your anti-Apg10 (Atg10) Rabbit Monoclonal antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
As indicated on the product datasheet, M07803 anti-Apg10 (Atg10) Rabbit Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
We are currently using anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
The anti-Apg10 (Atg10) Rabbit Monoclonal antibody (M07803) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-21
Question
Will anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 work for WB with testis?
C. Banerjee
Verified customer
Asked: 2014-05-28
Answer
According to the expression profile of testis, ATG10 is highly expressed in testis. So, it is likely that anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 will work for WB with testis.
Boster Scientific Support
Answered: 2014-05-28
Question
Do you have a BSA free version of anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 available?
A. Collins
Verified customer
Asked: 2013-04-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Apg10 (Atg10) Rabbit Monoclonal antibody M07803 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-10
