Anti-APOBEC3B Antibody

Product Name

Anti-APOBEC3B Antibody

View all APOBEC3B Antibodies

SKU/Catalog Number

A01088

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-APOBEC3B Antibody (Catalog # A01088). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

APOBEC3B antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-APOBEC3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01088)

Host

Rabbit

Contents

APOBEC3B Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B. The immunogen is located within amino acids 20 - 70 of APOBEC3B.

Cross-reactivity

APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.

Reactive Species

A01088 is reactive to APOBEC3B in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.9 kDa

Background of APOBEC3B

The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers; it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01088 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information APOBEC3B

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