Anti-APOBEC3B Antibody Picoband™

Product Name

Anti-APOBEC3B Antibody Picoband™

View all APOBEC3B Antibodies

SKU/Catalog Number

A01088-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-APOBEC3B Antibody Picoband™ catalog # A01088-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-APOBEC3B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01088-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human APOBEC3B (FNNDPLVLRRRQTYLCYEVER).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01088-1 is reactive to APOBEC3B in Human, Mouse, Rat

Applications

A01088-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Background of APOBEC3B

Probable DNA dC->dU-editing enzyme APOBEC-3B is a protein that in humans is encoded by the APOBEC3B gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/ml/1x10^6 cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of APOBEC3B using anti-APOBEC3B antibody (A01088-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3B antigen affinity purified polyclonal antibody (Catalog # A01088-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3B at approximately 46 kDa. The expected band size for APOBEC3B is at 46 kDa.

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Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-APOBEC3B antibody (A01088-1).
Overlay histogram showing CACO-2 cells stained with A01088-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APOBEC3B Antibody (A01088-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For APOBEC3B (Source: Uniprot.Org, NCBI)

Alternative Names

APOBEC1L; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B; ARCD3; ARP4; bK150C2.2; cytidine deaminase; DJ742C19.2; EC 3.5.4; EC 3.5.4.-; FLJ21201; phorbolin 3; Phorbolin-1-related protein; phorbolin-2/3; PHRBNLphorbolin 2; probable DNA dC->dU-editing enzyme APOBEC-3B APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2 apolipoprotein B mRNA editing enzyme catalytic subunit 3B DNA dC->dU-editing enzyme APOBEC-3B|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B|cytidine deaminase|phorbolin 2|phorbolin 3|phorbolin-1-related protein|phorbolin-2/3|probable DNA dC->dU-editing enzyme APOBEC-3B

For more info on APOBEC3B, check out the APOBEC3B Infographic

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In this infographic you will see the following information for APOBEC3B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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