Anti-APOBEC3G Antibody Picoband™

Product Name

Anti-APOBEC3G Antibody Picoband™

View all APOBEC3G Antibodies

SKU/Catalog Number

PB9985

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-APOBEC3G Antibody Picoband™ catalog # PB9985. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APOBEC3G Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9985)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9985 is reactive to APOBEC3G in Human

Applications

PB9985 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

46.408kDa

Background of APOBEC3G

APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3G antigen affinity purified polyclonal antibody (Catalog # PB9985) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 40 kDa. The expected band size for APOBEC3G is at 46 kDa.

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Figure 2. IHC analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985).
APOBEC3G was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOBEC3G Antibody (PB9985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985).
APOBEC3G was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOBEC3G Antibody (PB9985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of THP-1 cells using anti-APOBEC3G antibody (PB9985).
Overlay histogram showing THP-1 cells stained with PB9985 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APOBEC3G Antibody (PB9985,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For APOBEC3G (Source: Uniprot.org, NCBI)

Alternative Names

APOBEC-related cytidine deaminase; APOBEC-related protein 9; APOBEC-related protein; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; ARCD; ARP-9; bK150C2.7; CEM-15; CEM15ARP9; dJ494G10.1; DNA dC->dU-editing enzyme APOBEC-3G; EC 3.5.4; EC 3.5.4.-; EC 3.5.4.5; FLJ12740DNA dC->dU editing enzyme; MDS019; phorbolin-like protein MDS019 APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1 apolipoprotein B mRNA editing enzyme catalytic subunit 3G DNA dC->dU-editing enzyme APOBEC-3G|APOBEC-related cytidine deaminase|APOBEC-related protein 9|DNA dC->dU editing enzyme|apolipoprotein B editing enzyme catalytic polypeptide-like 3G|apolipoprotein B mRNA editing enzyme cytidine deaminase|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G|apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G|deoxycytidine deaminase|phorbolin-like protein MDS019

For more info on APOBEC3G, check out the APOBEC3G Infographic

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In this infographic, you will see the following information for APOBEC3G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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