Anti-C-C chemokine receptor type 5 CCR5 Antibody

Boster Bio Anti-C-C chemokine receptor type 5 CCR5 Antibody catalog # PA1016-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PA1016-1
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-C-C chemokine receptor type 5 CCR5 Antibody

See all CCR5 products

SKU/Catalog Number

PA1016-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-C-C chemokine receptor type 5 CCR5 Antibody catalog # PA1016-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C-C chemokine receptor type 5 CCR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1016-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human CCR5 (179-197aa SSHFPYSQYQFWKNFQTLK).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1016-1 is reactive to CCR5 in Human

Applications

PA1016-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CCR5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P51681

Gene ID

1234

Gene Name

CCR5

Full Name

C-C chemokine receptor type 5

Weight

40.524kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5 CCR5 CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22 C-C motif chemokine receptor 5 C-C chemokine receptor type 5|C-C motif chemokine receptor 5 A159A|HIV-1 fusion coreceptor|chemokine (C-C motif) receptor 5|chemokine receptor CCR5|chemokine recptor CCR5 Delta32|chemr13

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCR5, check out the CCR5 Infographic

CCR5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCR5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1016-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Study on CCR5 analogs and affinity peptides

Li G, Xu H, Zhu S, Xu W, Qin S, Liu S, Tu G, Peng H, Qiu S, Yu S, Zhu Q, Fan B, Zheng C, Li G, Liang S. Brain Res Bull. 2013 Jan;90:79-87. Doi: 10.1016/J.Brainresbull.2012.10.002. Epub 2012 Oct 9. Effects Of Neferine On Ccl5 And Ccr5 Expression In...

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5 Customer Q&As for Anti-C-C chemokine receptor type 5 CCR5 Antibody

Question

We have observed staining in human visceral pleura. Are there any suggestions? Is anti-CCR5 antibody supposed to stain visceral pleura positively?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

Based on literature visceral pleura does express CCR5. Based on Uniprot.org, CCR5 is expressed in visceral pleura, macrophage, among other tissues. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314

Boster Scientific Support

Answered: 2019-08-21

Question

Our lab want to know about using your anti-CCR5 antibody for chemotaxis studies. Has this antibody been tested with western blotting on smmc cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We appreciate your inquiry. This PA1016-1 anti-CCR5 antibody is tested on colo320 cell lysate, smmc cell lysate, jurkat cell lysate. It is guaranteed to work for Flow Cytometry, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-23

Question

We were happy with the WB result of your anti-CCR5 antibody. However we have observed positive staining in macrophage cell membrane using this antibody. Is that expected? Could you tell me where is CCR5 supposed to be expressed?

B. Roberts

Verified customer

Asked: 2018-10-05

Answer

From what I have seen in literature, macrophage does express CCR5. Generally CCR5 expresses in cell membrane. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314

Boster Scientific Support

Answered: 2018-10-05

Question

We ordered your anti-CCR5 antibody for WB on visceral pleura in the past. I am using human, and We intend to use the antibody for Flow Cytometry next. I would like examining visceral pleura as well as macrophage in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2018-09-24

Answer

I viewed the website and datasheets of our anti-CCR5 antibody and it appears that PA1016-1 has been tested on human in both WB and Flow Cytometry. Thus PA1016-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-09-24

Question

We are currently using anti-CCR5 antibody PA1016-1 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

K. Dhar

Verified customer

Asked: 2017-04-25

Answer

The anti-CCR5 antibody (PA1016-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-04-25

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1016-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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