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Product Info Summary
| SKU: | A09879 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ApoO Monoclonal Antibody
SKU/Catalog Number
A09879
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ApoO Monoclonal Antibody catalog # A09879. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ApoO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09879)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2F1
Isotype
IgG
Immunogen
Purified recombinant fragment of ApoO expressed in E. Coli.
Reactive Species
A09879 is reactive to APOO in Human, Mouse
Calculated molecular weight
22.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09879 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis using ApoO Monoclonal Antibody against HepG2 (1) and 3T3L1 (2) cell lysate
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Immunohistochemistry analysis of paraffin-embedded human Testis tissues with AEC staining using ApoO Monoclonal Antibody.
Specific Publications For Anti-ApoO Monoclonal Antibody (A09879)
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Customer Q&As
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7 Customer Q&As for Anti-ApoO Monoclonal Antibody
Question
I see that the anti-ApoO Monoclonal antibody A09879 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-ApoO Monoclonal antibody A09879 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
P. Mangal
Verified customer
Asked: 2020-01-07
Answer
The anti-ApoO Monoclonal antibody (A09879) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
Question
I have attached the WB image, lot number and protocol we used for skin urinary bladder using anti-ApoO Monoclonal antibody A09879. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin urinary bladder using anti-ApoO Monoclonal antibody A09879. Let me know if you need anything else.
W. Moore
Verified customer
Asked: 2019-09-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
Does anti-ApoO Monoclonal antibody A09879 work on bovine IHC with brain?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not validated anti-ApoO Monoclonal antibody A09879 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ApoO Monoclonal antibody A09879 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
My question regarding product A09879, anti-ApoO Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09879 anti-ApoO Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-10
Question
I was wanting to use your anti-ApoO Monoclonal antibody for WB for human skin urinary bladder on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin urinary bladder identification?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
As indicated on the product datasheet, A09879 anti-ApoO Monoclonal antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human skin urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-19
