Anti-Apoptosis-inducing factor 3 AIFM3 Antibody

Product Name

Anti-Apoptosis-inducing factor 3 AIFM3 Antibody

View all AIFM3 Antibodies

SKU/Catalog Number

A11286

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Apoptosis-inducing factor 3 AIFM3 Antibody (Catalog # A11286). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

AIFM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Apoptosis-inducing factor 3 AIFM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11286)

Host

Rabbit

Contents

AIFM3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AIFM3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human AIFM3. The immunogen is located within the first 50 amino acids of AIFM3.

Reactive Species

A11286 is reactive to AIFM3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

66.8 kDa

Background of AIFM3

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11286 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AIFM3

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