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Product Info Summary
| SKU: | A06828-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Aprataxin and PNK-like factor APLF Antibody
SKU/Catalog Number
A06828-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Aprataxin and PNK-like factor APLF Antibody catalog # A06828-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aprataxin and PNK-like factor APLF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06828-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human APLF around the non-phosphorylation site of S116.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06828-2 is reactive to Aplf in Human, Mouse
Applications
A06828-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.968kDa
Background of Aplf
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of APLF using Anti-Aprataxin and PNK-like factor APLF Antibody (A06828-2).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C
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Figure 1. Western blotting validation for Anti-Aprataxin and PNK-like factor APLF Antibody A06828-2
Western Blot (WB) analysis of HT-29 cells using APLF polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Aplf (Source: Uniprot.org, NCBI)
Gene Name
Aplf
Full Name
Aprataxin and PNK-like factor
Weight
54.968kDa
Superfamily
APLF family
Alternative Names
APFL; aprataxin- and PNK-like factor; aprataxin and PNK-like factor; aprataxin and PNKP like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13FLJ16593; chromosome 2 open reading frame 13; EC 4.2.99.18; MGC47799; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; Xip1; XRCC1-interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aplf, check out the Aplf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aplf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aprataxin and PNK-like factor APLF Antibody (A06828-2)
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No publications found for A06828-2
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