Anti-Aquaporin 8/AQP8 Antibody

Product Name

Anti-Aquaporin 8/AQP8 Antibody

View all Aquaporin-8 Antibodies

SKU/Catalog Number

PA1511

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aquaporin 8/AQP8 Antibody catalog # PA1511. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 8/AQP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1511)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Aquaporin 8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1511 is reactive to AQP8 in Human, Mouse, Rat

Applications

PA1511 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

27.381kDa

Background of Aquaporin-8

AQP8, also known as Aquaporin-8, is a protein that in humans is encoded by the AQP8 gene. Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. The AQP8 gene contains 6 exons. It is mapped to chromosome 16p12. Northern blot analysis of human tissues detected a 1.35-kb AQP8 mRNA only in pancreas and colon.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.

Click image to see more details

Figure 2. Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.

Gene/Protein Information For AQP8 (Source: Uniprot.org, NCBI)

Alternative Names

AQP-8; aquaporin 8; aquaporin-8 AQP8 AQP-8 aquaporin 8 aquaporin-8

For more info on AQP8, check out the AQP8 Infographic

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In this infographic, you will see the following information for AQP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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