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- Table of Contents
ALOX15 antibody (PB9073) performs well in IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband®
ALOX15 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
ALOX15 antibody was positively detected by WB in:
rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALOX15 antibody was positively detected by IHC in:
mouse lung tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Mouse
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband®
View all ALOX15 Antibodies
View all ALOX15 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
74.8 kDa
Background of ALOX15
Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.
Cite This Product
Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9073)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Cow
86%
Moderate Homology
Sheep
85%
Moderate Homology
Dog
82%
Moderate Homology
Rat
73%
Moderate Homology
Mouse
72%
Moderate Homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ALOX15 antibody
Immunohistochemistry (IHC) protocol for ALOX15 antibody
Publications
Application:
Species:
Dietary Limonin ameliorates heart failure with preserved ejection fraction by targeting ferroptosis via modulation of the Nrf2/SLC7A11/GPX4 axis: An integrated transcriptomics and metabolomics analysis
Published: 2025-03-31
Journal: Food & Function
Application: IF,WB
Impact Factor: 5.40
Species: Mouse
PMID: 40230319
Se-Methylselenocysteine Ameliorates DEHP-Induced Ferroptosis in Testicular Sertoli Cells via the Nrf2/GPX4 Axis
Published: 2024-10-03
Journal: ENVIRONMENTAL TOXICOLOGY
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 39360521
AQP4-AS1 Can Regulate the Expression of Ferroptosis-Related Regulator ALOX15 through Competitive Binding with miR-4476 in Lung Adenocarcinoma
Published: 2024-08-16
Journal: Global Medical Genetics
Application: WB
Impact Factor: 1.20
Species: Human
PMID: 39155888
Review
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Product Promise
We promise Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026