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Product Info Summary
| SKU: | A04634 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ARF-BP1 HUWE1 Antibody
SKU/Catalog Number
A04634
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ARF-BP1 HUWE1 Antibody (Catalog # A04634). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ARF-BP1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ARF-BP1 HUWE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04634)
Host
Rabbit
Contents
ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1.
Reactive Species
A04634 is reactive to HUWE1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
481.9 kDa
Background of HUWE1
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04634 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/mL.
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Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/mL.
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Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 μg/mL.
Specific Publications For Anti-ARF-BP1 HUWE1 Antibody (A04634)
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