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Product Info Summary
| SKU: | A06958 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ARHGEF10 Antibody
SKU/Catalog Number
A06958
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ARHGEF10 Antibody catalog # A06958. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARHGEF10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06958)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF10. AA range:339-388
Reactive Species
A06958 is reactive to ARHGEF10 in Human, Monkey
Observed Molecular Weight
39 kDa
Calculated molecular weight
151.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06958 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using ARHGEF10 Polyclonal antibody.
Specific Publications For Anti-ARHGEF10 Antibody (A06958)
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Customer Q&As
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4 Customer Q&As for Anti-ARHGEF10 Antibody
Question
Will anti-ARHGEF10 antibody A06958 work on dog WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Our lab technicians have not validated anti-ARHGEF10 antibody A06958 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ARHGEF10 antibody A06958 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
Does A06958 anti-ARHGEF10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
As indicated on the product datasheet, A06958 anti-ARHGEF10 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-11
Question
Please see the WB image, lot number and protocol we used for tibial nerve using anti-ARHGEF10 antibody A06958. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-21
Question
We are currently using anti-ARHGEF10 antibody A06958 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey. Is it true that the antibody can work on horse tissues as well?
K. Baker
Verified customer
Asked: 2013-01-31
Answer
The anti-ARHGEF10 antibody (A06958) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-31
