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ARID3A antibody (A03604-1) performs well in Flow Cytometry, IP, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Figure2. Flow Cytometry analysis of Caco-2 cells using anti-ARID3A antibody (A03604-1).
Overlay histogram showing Caco-2 cells stained with A03604-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID3A Antibody (A03604-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Caco-2 cells stained with A03604-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID3A Antibody (A03604-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ARID3A Antibody Picoband®
ARID3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, WB |
Tested Applications
ARID3A antibody was positively detected by WB in:
human K562 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ARID3A antibody was positively detected by FC in:
Caco-2 cells
ARID3A antibody was positively detected by IP in:
HepG2 cells
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Mouse
Predicted
Rat
Predicted
Product Information
Product Name
Anti-ARID3A Antibody Picoband®
View all ARID3A Antibodies
View all ARID3A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A, which shares 95.8% amino acid (aa) sequence identity with mouse ARID3A.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
62.9 kDa
Background of ARID3A
AT-rich interactive domain-containing protein 3A is a protein that in humans is encoded by the ARID3A gene. This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.
Cite This Product
Anti-ARID3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03604-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Zebrafish
67%
Low homology
Drosophila
13%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ARID3A antibody
Flow Cytometry (FC) protocol for ARID3A antibody
Immunoprecipitation (IP) protocol for ARID3A antibody
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Product Promise
We promise Anti-ARID3A Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03604-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026