Click image to see more details
Product Info Summary
| SKU: | A12854 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ARP5/ACTR5 Antibody
SKU/Catalog Number
A12854
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ARP5/ACTR5 Antibody catalog # A12854. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-ARP5/ACTR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12854)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human ARP5/ACTR5 recombinant protein (Position: L260-G604).
Reactive Species
A12854 is reactive to ACTR5 in Human, Mouse, Rat
Calculated molecular weight
68.3 kDa
Background of ACTR5
Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12854 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 1:500-2000 | |
| Immunocytochemistry/Immunofluorescence | 1:50-400 | |
| ImmunoPrecipitation | 1:250-300 | |
| ELISA | 1:100-1000 |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ARP5/ACTR5 using anti-ARP5/ACTR5 antibody (A12854).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARP5/ACTR5 antigen affinity purified polyclonal antibody (A12854) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARP5/ACTR5 at approximately 68 kDa. The expected band size for ARP5/ACTR5 is at 68 kDa.
Specific Publications For Anti-ARP5/ACTR5 Antibody (A12854)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ARP5/ACTR5 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ARP5/ACTR5 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
