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- Table of Contents
PYCARD antibody (A00362) performs well in ELISA, IHC-P, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of ASC in Human Spleen Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with A00362 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with A00362 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-ASC PYCARD Antibody
PYCARD antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL; ICC: 5 μg/mL; IHC-P: 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ASC PYCARD Antibody
View all PYCARD Antibodies
View all PYCARD Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
ASC antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
ASC Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-ASC antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
21.6 kDa
Background of PYCARD
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
Cite This Product
Anti-ASC PYCARD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00362)
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Product Promise
We promise Anti-ASC PYCARD Antibody performs excellently in relevant applications for ELISA, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00362
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026