Anti-ASPP1 Ppp1r13b Antibody

Product Name

Anti-ASPP1 Ppp1r13b Antibody

View all ASPP1 Antibodies

SKU/Catalog Number

A07257

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ASPP1 Ppp1r13b Antibody (Catalog # A07257). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ASPP1 Ppp1r13b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07257)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07257 is reactive to Ppp1r13b in Human

Applications

A07257 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

119.17kDa

Background of ASPP1

ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21.  Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of ASPP1 expression in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. ASPP1 was detected using rabbit anti-ASPP1 Antigen Affinity purified polyclonal antibody (Catalog # A07257) at 1:1000. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.

Gene/Protein Information For Ppp1r13b (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis-stimulating protein of p53, 1; ASPP1Protein phosphatase 1 regulatory subunit 13B; KIAA0771apoptosis-stimulating of p53 protein 1; p53BP2-like; p85; protein phosphatase 1, regulatory (inhibitor) subunit 13B Ppp1r13b|A, AI449786, ASPP1, AW545810, Tp53bp2, Trp53bp2, p85|protein phosphatase 1, regulatory subunit 13B|apoptosis-stimulating of p53 protein 1|protein phosphatase 1, regulatory (inhibitor) subunit 13B|transformation related protein 53 binding protein 2|tumor protein p53 binding protein, 2

For more info on Ppp1r13b, check out the Ppp1r13b Infographic

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In this infographic, you will see the following information for Ppp1r13b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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