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Product Info Summary
| SKU: | A02333 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HUMAN-CTNND1_isform 2ABC(Y174) Antibody
SKU/Catalog Number
A02333
Size
80 µl
Description
Boster Bio Anti-HUMAN-CTNND1_isform 2ABC(Y174) Antibody (Catalog # A02333). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HUMAN-CTNND1_isform 2ABC(Y174) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02333)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 160-200 amino acids from human.
Reactive Species
A02333 is reactive to CTNND1 in Human
Calculated molecular weight
108.2 kDa
Background of CTNND1
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02333 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-HUMAN-CTNND1_isform 2ABC (Y174) Antibody at 1:16000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: MDA-MB-231 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 108 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-HUMAN-CTNND1_isform 2ABC (Y174) Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 108 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HUMAN-CTNND1_isform 2ABC(Y174) Antibody (A02333)
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