Product Info Summary
SKU: | M01786 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Ataxin-1 ATXN1 Monoclonal Antibody
SKU/Catalog Number
M01786
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ataxin-1 ATXN1 Monoclonal Antibody catalog # M01786. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ataxin-1 ATXN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01786)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2F5
Isotype
IgG
Immunogen
Purified recombinant fragment of human Ataxin-1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01786 is reactive to ATXN1 in Human
Applications
M01786 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
86.923kDa
Background of Ataxin 1
Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. Flow Cytometry validation of ATXN1 using Anti-Ataxin-1 ATXN1 Monoclonal Antibody (M01786).
Flow cytometric (FCM) analysis of Jurkat cells using Ataxin-1 Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 3. Immunofluorescent staining data of ATXN1 using Anti-Ataxin-1 ATXN1 Monoclonal Antibody (M01786).
Immunofluorescence (IF) analysis of NTERA-2 cells using Ataxin-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of ATXN1 using Anti-Ataxin-1 ATXN1 Monoclonal Antibody (M01786).
Immunohistochemistry (IHC) analysis of paraffin-embedded ovarian cancer tissues (left) and Lung Cancer Tissues (right) with DAB staining using Ataxin-1 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Ataxin-1 ATXN1 Monoclonal Antibody M01786
Western Blot (WB) analysis using Ataxin-1 Monoclonal Antibody against HEK293 (1) and ATXN1-hIgGFc transfected HEK293 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ATXN1 (Source: Uniprot.org, NCBI)
Gene Name
ATXN1
Full Name
Ataxin-1
Weight
86.923kDa
Superfamily
ATXN1 family
Alternative Names
ataxin 1; ataxin 1); ataxin-1; ATX1spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant; SCA1D6S504E; Spinocerebellar ataxia type 1 protein ATXN1 ATX1, D6S504E, SCA1 ataxin 1 ataxin-1|alternative ataxin1|spinocerebellar ataxia type 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATXN1, check out the ATXN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATXN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ataxin-1 ATXN1 Monoclonal Antibody (M01786)
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No publications found for M01786
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Customer Reviews
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