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Product Info Summary
| SKU: | A07459-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ATE1 Antibody Picoband™
SKU/Catalog Number
A07459-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATE1 Antibody Picoband™ catalog # A07459-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ATE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07459-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ATE1 recombinant protein (Position: R56-A459).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07459-1 is reactive to ATE1 in Human
Applications
A07459-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.09kDa
Background of ATE1
This gene encodes an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartate or glutamate targets proteins for ubiquitin-dependent degradation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATE1 using anti-ATE1 antibody (A07459-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATE1 antigen affinity purified polyclonal antibody (Catalog # A07459-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATE1 at approximately 59 kDa. The expected band size for ATE1 is at 59 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ATE1 (Source: Uniprot.org, NCBI)
Gene Name
ATE1
Full Name
Arginyl-tRNA--protein transferase 1
Weight
59.09kDa
Superfamily
R-transferase family
Alternative Names
Arginine-tRNA--protein transferase 1; arginyltransferase 1MGC26724; arginyl-tRNA--protein transferase 1; arginyl-tRNA-protein transferase; EC 2.3.2.8; R-transferase 1 ATE1 arginyltransferase 1 arginyl-tRNA--protein transferase 1|R-transferase 1|arginine-tRNA--protein transferase 1|arginyl-tRNA-protein transferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATE1, check out the ATE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATE1 Antibody Picoband™ (A07459-1)
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No publications found for A07459-1
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Customer Reviews
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