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Product Info Summary
| SKU: | A00904 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ATF3 Antibody
SKU/Catalog Number
A00904
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ATF3 Antibody (Catalog # A00904). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ATF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00904)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.
Reactive Species
A00904 is reactive to ATF3 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
20.6 kDa
Background of ATF3
ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00904 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
WB: 1:200 - 1:2000
Affinity purified anti-ATF3 has been tested by ELISA and western blotting against recombinant forms of the protein. Although not tested, this antibody is likely function in most immunoassays including immunofluorescence microscopy, immunohistochemistry.
Validation Images & Assay Conditions
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Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Boster's Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using Boster's anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Boster's Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Boster's anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Specific Publications For Anti-ATF3 Antibody (A00904)
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7 Customer Q&As for Anti-ATF3 Antibody
Question
Does anti-ATF3 antibody A00904 work for WB with vena cava?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
According to the expression profile of vena cava, ATF3 is highly expressed in vena cava. So, it is likely that anti-ATF3 antibody A00904 will work for WB with vena cava.
Boster Scientific Support
Answered: 2020-03-27
Question
Will anti-ATF3 antibody A00904 work on zebrafish WB with cerebellum lung?
W. Parker
Verified customer
Asked: 2019-02-13
Answer
Our lab technicians have not tested anti-ATF3 antibody A00904 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ATF3 antibody A00904 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cerebellum lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-13
Question
Do you have a BSA free version of anti-ATF3 antibody A00904 available?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATF3 antibody A00904 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-01
Question
Is this A00904 anti-ATF3 antibody reactive to the isotypes of ATF3?
M. Brown
Verified customer
Asked: 2017-01-19
Answer
The immunogen of A00904 anti-ATF3 antibody is This antibody was produced from a synthetic peptide corresponding to aa 113-130 of human ATF3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-01-19
Question
I have attached the WB image, lot number and protocol we used for vena cava using anti-ATF3 antibody A00904. Please let me know if you require anything else.
D. Williams
Verified customer
Asked: 2014-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-08
Question
I am interested in to test anti-ATF3 antibody A00904 on human vena cava for research purposes, then I may be interested in using anti-ATF3 antibody A00904 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Miller
Verified customer
Asked: 2014-03-03
Answer
The products we sell, including anti-ATF3 antibody A00904, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-03
Question
We are currently using anti-ATF3 antibody A00904 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
O. Krishna
Verified customer
Asked: 2013-07-02
Answer
The anti-ATF3 antibody (A00904) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-02
