Anti-ATG12 Antibody Picoband™

Product Name

Anti-ATG12 Antibody Picoband™

View all ATG12 Antibodies

SKU/Catalog Number

A00820-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-ATG12 Antibody Picoband™ catalog # A00820-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ATG12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00820-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ATG12 recombinant protein (Position: M68-Q99).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00820-2 is reactive to ATG12 in Human

Applications

A00820-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

15.113kDa

Background of ATG12

Autophagy related 12 is a protein that in humans is encoded by the ATG12 gene. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ATG12 using anti-ATG12 antibody (A00820-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human COLO 320 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG12 antigen affinity purified polyclonal antibody (Catalog # A00820-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG12 at approximately 48 kDa. The expected band size for ATG12 is at 15 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-ATG12 antibody (A00820-2).
Overlay histogram showing RT4 cells stained with A00820-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG12 Antibody (A00820-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ATG12 (Source: Uniprot.org, NCBI)

Alternative Names

Apg12 (autophagy 12, S. cerevisiae)-like; APG12 autophagy 12-like (S. cerevisiae); APG12; APG12HAPG12; APG12-like; ATG12 autophagy related 12 homolog (S. cerevisiae); ATG12; Autophagy-related protein 12; FBR93; HAPG12; ubiquitin-like protein ATG12; yeast) homolog ATG12 APG12, APG12L, FBR93, HAPG12 autophagy related 12 ubiquitin-like protein ATG12|APG12 autophagy 12-like|ATG12 autophagy related 12 homolog|Apg12 (autophagy, yeast) homolog

For more info on ATG12, check out the ATG12 Infographic

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In this infographic, you will see the following information for ATG12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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