Anti-ATP1A3 Rabbit Monoclonal Antibody

Product Name

Anti-ATP1A3 Rabbit Monoclonal Antibody

View all Sodium Potassium ATPase Alpha 3 Antibodies

SKU/Catalog Number

M02278

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-ATP1A3 Rabbit Monoclonal Antibody catalog # M02278. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP1A3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02278)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

22A98

Isotype

IgG

Immunogen

A synthesized peptide derived from human ATP1A3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02278 is reactive to ATP1A3 in Human, Mouse, Rat

Applications

M02278 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

100-150 kDa

Calculated molecular weight

111.749kDa

Background of Sodium Potassium ATPase Alpha 3

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (M02278).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified monoclonal antibody (Catalog # M02278) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 112 kDa. The expected band size for ATP1A3 is at 112 kDa.

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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Western blot analysis of ATP1A3 expression in Rat brain lysate.

Gene/Protein Information For ATP1A3 (Source: Uniprot.org, NCBI)

Alternative Names

alpha 3 polypeptide; ATPase, Na+/K+ transporting, alpha 3 polypeptide; dystonia 12; DYT12; EC 3.6.3; MGC13276; RDP; Sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3 ATP1A3 AHC2, ATP1A1, CAPOS, DYT12, RDP ATPase Na+/K+ transporting subunit alpha 3 sodium/potassium-transporting ATPase subunit alpha-3|ATPase, Na+/K+ transporting, alpha 3 polypeptide|Na(+)/K(+) ATPase alpha(III) subunit|Na(+)/K(+) ATPase alpha-3 subunit|Na+, K+ activated adenosine triphosphatase alpha subunit|Na+/K+ ATPase 3|sodium pump subunit alpha-3|sodium-potassium ATPase catalytic subunit alpha-3|sodium-potassium-ATPase, alpha 3 polypeptide|sodium/potassium-transporting ATPase alpha-3 chain

For more info on ATP1A3, check out the ATP1A3 Infographic

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In this infographic, you will see the following information for ATP1A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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