Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

Product Name

Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

View all ATP5A1 Antibodies

SKU/Catalog Number

M03598
BM5043 is an alternative SKU for this antibody, used in previous lots.

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody catalog # M03598. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03598)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AAFC-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ATP5A1

Reactive Species

M03598 is reactive to ATP5A1 in Human, Mouse, Rat

Observed Molecular Weight

50 kDa

Calculated molecular weight

59.8 kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03598 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Recommend Dilution

WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20

Tested application

WB:human HepG2 whole cell, human RT4 whole cell, human HEL whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue

Validation Images & Assay Conditions

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Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (M03598).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified monoclonal antibody (M03598) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 50 kDa. The expected band size for ATP5F1A is at 60 kDa.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.