Product Info Summary
SKU: | A32374-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ATP5F1 Antibody (Center)
SKU/Catalog Number
A32374-1
Size
80 µl
Description
Boster Bio Anti-ATP5F1 Antibody (Center) (Catalog # A32374-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP5F1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32374-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the Central region of human ATP5F1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32374-1 is reactive to ATP5PB in Human, Mouse, Rat
Applications
A32374-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
28.909kDa
Background of ATP5F1
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) -containing the extramembraneous catalytic core, and F(0) -containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
ATP5F1 Antibody (Center) western blot analysis in HepG2,HL-60 cell line,mouse kidney and rat heart tissue lysates (35ug/lane).This demonstrates the ATP5F1 antibody detected the ATP5F1 protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For ATP5PB (Source: Uniprot.org, NCBI)
Gene Name
ATP5PB
Full Name
ATP synthase F(0) complex subunit B1, mitochondrial
Weight
28.909kDa
Superfamily
eukaryotic ATPase B chain family
Alternative Names
ATP synthase B chain, mitochondrial; ATP synthase subunit b, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATPase subunit b; cell proliferation-inducing protein 47; H+-ATP synthase subunit b; MGC24431; PIG47 ATP5PB ATP5F1, PIG47 ATP synthase peripheral stalk-membrane subunit b ATP synthase F(0) complex subunit B1, mitochondrial|ATP synthase B chain, mitochondrial|ATP synthase proton-transporting mitochondrial F(0) complex subunit B1|ATP synthase subunit b, mitochondrial|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b|ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1|ATPase subunit b|H+-ATP synthase subunit b|cell proliferation-inducing protein 47
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5PB, check out the ATP5PB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5PB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5F1 Antibody (Center) (A32374-1)
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No publications found for A32374-1
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