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Product Info Summary
| SKU: | A32374-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATP5F1 Antibody (Center)
SKU/Catalog Number
A32374-1
Size
80 µl
Description
Boster Bio Anti-ATP5F1 Antibody (Center) (Catalog # A32374-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP5F1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32374-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the Central region of human ATP5F1.
Reactive Species
A32374-1 is reactive to ATP5PB in Human, Mouse, Rat
Calculated molecular weight
28.9 kDa
Background of ATP5PB
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) -containing the extramembraneous catalytic core, and F(0) -containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32374-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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ATP5F1 Antibody (Center) western blot analysis in HepG2,HL-60 cell line,mouse kidney and rat heart tissue lysates (35ug/lane).This demonstrates the ATP5F1 antibody detected the ATP5F1 protein (arrow).
Specific Publications For Anti-ATP5F1 Antibody (Center) (A32374-1)
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