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Product Info Summary
| SKU: | A32370 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-ATP5I (K69) ATP5ME Antibody
SKU/Catalog Number
A32370
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATP5I (K69) ATP5ME Antibody catalog # A32370. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5I (K69) ATP5ME Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32370)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human ATP5I.
Reactive Species
A32370 is reactive to ATP5ME in Human, Mouse
Calculated molecular weight
7.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32370 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP 1:50-1:100
Validation Images & Assay Conditions
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Western blot (WB) analysis of ATP5I (K69) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of ATP5I (K69) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunoprecipitation of HEK293T cell lysate using ATP5I (K69) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using ATP5I (K69) #BS3813. “↑”（supernatant）; “↓(deposition）
Specific Publications For Anti-ATP5I (K69) ATP5ME Antibody (A32370)
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