Click image to see more details
Product Info Summary
| SKU: | A07713 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Autophagy-related protein 101 ATG101 Antibody
SKU/Catalog Number
A07713
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Autophagy-related protein 101 ATG101 Antibody (Catalog # A07713). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG101 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Autophagy-related protein 101 ATG101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07713)
Host
Rabbit
Contents
ATG101 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG101 antibody was raised against a 16 amino acid synthetic peptide near the center of human ATG101. The immunogen is located within amino acids 80 - 130 of ATG101.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.
Reactive Species
A07713 is reactive to ATG101 in Human, Mouse, Rat
Applications
A07713 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.003kDa
Background of ATG101
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 μg/mL.
Click image to see more details
Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG101 (Source: Uniprot.org, NCBI)
Gene Name
ATG101
Full Name
Autophagy-related protein 101
Weight
25.003kDa
Superfamily
ATG101 family
Alternative Names
Autophagy-related protein 101 ATG101 C12orf44 autophagy related 101 autophagy-related protein 101|Atg13-interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG101, check out the ATG101 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG101: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Autophagy-related protein 101 ATG101 Antibody (A07713)
Hello CJ!
No publications found for A07713
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Autophagy-related protein 101 ATG101 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Autophagy-related protein 101 ATG101 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
